Coding

Part:BBa_K3513003:Design

Designed by: Huandi Xu   Group: iGEM20_NFLS   (2020-10-23)


exoglucanase with signal peptide


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 596
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 61
    Illegal NgoMIV site found at 229
    Illegal NgoMIV site found at 602
    Illegal NgoMIV site found at 1104
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 649
    Illegal SapI.rc site found at 732


Design Notes

Add a OmpA signal peptide to make it secret out of the cells.


Source

Origin source of endoglucanase: Streptomyces Coelicolor. But the team engineered it.


References